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The R-PLEX Human Heme Oxygenase 1 Assay Kit from Meso Scale Discovery is a multiplex immunoassay that measures Heme Oxygenase 1 (HO-1) in human serum plasma and cell culture supernatants The assay uses electrochemiluminescence (ECL) detection technology and provides high sensitivity broad dynamic range and intra- and inter-assay precision This kit includes a 96-well plate coated with capture antibody detection antibody Read Buffer and calibrator It can test up to 80 samples in duplicate and only requires a small sample volume of 50 L The assay takes around 3 5 hours to complete
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In Western blotting this antibody recognizes proteins in MW range of 265-400 kDa identified as different glycoforms of EMA This MAb reacts with the DTRP epitope in the tandem repeats Thealpha subunit has cell adhesive properties It can act both as an adhesion and an anti-adhesion protein EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack The beta subunit contains a C-terminal domain which is involved in cell signaling through phosphorylations and protein-protein interactions In immunohistochemical assays it superbly stains routine formalin/paraffin carcinoma tissues Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver Primary antibodies are available purified or with a selection of fluorescent CF Dyes and other labels CF Dyes offer exceptional brightness and photostability Note Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low
Encompass Procurement Services Non-distribution item offered as a customer accommodation; additional freight charges may apply. Learn More